Engineering genetic information requires having a good understanding of the physical structuring of genomes. In bacteria, this structuring is affected at multiple physical scales, from base pair pairing to the spatial organization of chromosome, by the underwinding of DNA – called negative supercoiling. In vivo DNA supercoiling thus plays a crucial role in multiple fundamental processes such as gpar ivan.junier@univ-grenoble-alpes.fr - Thèses hors de l'EDPIF